Contribution of B-lymphocytes to the production of Interleukin-17 in multiple sclerosis

نویسندگان

چکیده

The study of pathogenic systems involved in multiple sclerosis (MS) is essential for the development new therapies. Objective : to determine contribution B-lymphocytes production IL-17 MS with an assessment ability B-cells induce differentiation Th17 and own by this pathology. Material methods . A total 81 subjects were examined, 68 whom patients diagnosed MS, 13 healthy individuals. concentrations IL-17A, IL-10, BAFF IgG analyzed blood serum ELISA. additional included 11 donors. Mononuclear cells isolated from blood, which CD4+T-lymphocytes obtained. incubated tetanus toxoid or myelin-oligodendrocyte glycoprotein (MOG), after they washed unbounded antigen cultured together autologous peripheral mononuclear cells, then content sample was evaluated. Flow cytometry ex vivo assessed expression RORγt CD4+T-lymphocytes. level cell culture supernatants determined Results High levels are associated elevated concentrations, high levels, also twice as high. statistically significant higher IL-17-positive detected MS. Conclusion may contribute two ways – inducing T-lymphocytes producing cytokine their synthesis IL-17.

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ژورنال

عنوان ژورنال: Nevrologiâ, nejropsihiatriâ, psihosomatika

سال: 2023

ISSN: ['2310-1342', '2074-2711']

DOI: https://doi.org/10.14412/2074-2711-2023-1s-15-21